A spectrofluorometer is an analytical instrument used to measure the fluorescence emitted by a sample when it is excited by light. It works by shining a specific wavelength of light onto the sample, which then absorbs the energy and re-emits it at a longer wavelength. This emitted light is collected and analyzed to determine the concentration and properties of the fluorescent compounds present in the sample. These devices are widely used in various fields, including biochemistry, environmental science, and medical diagnostics. They help researchers and scientists study the behavior of molecules, detect pollutants, and analyze biological samples, making them essential tools in both research and industry.