Single-Particle Tracking (SPT) is a powerful imaging technique used to observe the movement of individual particles, such as proteins or nanoparticles, in real time. By using advanced microscopy methods, researchers can visualize how these particles move within cells or other environments, providing insights into their behavior and interactions. This technique is particularly valuable in fields like biophysics and cell biology, where understanding the dynamics of molecules is crucial. SPT allows scientists to gather quantitative data on particle trajectories, diffusion rates, and binding events, enhancing our knowledge of cellular processes and the mechanisms of diseases.