The Root Directory is the top-level folder in a file system, serving as the starting point for organizing files and subdirectories. It is often represented by a forward slash (/) in UNIX-like systems or a drive letter followed by a backslash (e.g., C:\) in Windows. All other files and folders branch out from this main directory, creating a hierarchical structure. In a computer, the Root Directory contains essential system files and directories, such as System32 in Windows or bin in UNIX. Understanding the root directory is crucial for navigating and managing files effectively within the operating system.