PRPP Synthetase is an enzyme that plays a crucial role in the synthesis of nucleotides, which are the building blocks of DNA and RNA. It catalyzes the conversion of ribose-5-phosphate and ATP into 5-phosphoribosyl-1-pyrophosphate (PRPP) and AMP. This reaction is essential for the production of purines and pyrimidines, which are vital for cellular functions. The activity of PRPP Synthetase is tightly regulated to ensure a balanced supply of nucleotides. Dysregulation of this enzyme can lead to various metabolic disorders, including gout and Lesch-Nyhan syndrome. Understanding its function is important for developing potential therapeutic strategies targeting nucleotide metabolism.