The "Not Equal To" symbol, represented as ≠, is used in mathematics and programming to indicate that two values are not the same. For example, if you have the numbers 5 and 3, you can say 5 ≠ 3, meaning that 5 is not equal to 3. This concept helps in comparing different quantities and making decisions based on their differences. In programming languages like Python or Java, the "Not Equal To" operator is often used in conditional statements to control the flow of a program. For instance, if a variable x is not equal to 10, the program can execute a specific block of code. This functionality is essential for creating logical conditions and ensuring accurate outcomes in software development.