Masson's trichrome is a histological staining technique used to differentiate between various tissue components in microscopic samples. It highlights collagen fibers in blue or green, muscle fibers in red, and cytoplasm in pink. This contrast helps pathologists identify tissue structure and abnormalities. The staining process involves multiple steps, including fixation, staining, and dehydration. It is particularly useful in studying connective tissues and diagnosing conditions such as fibrosis or tumors. By providing clear visual distinctions, Masson's trichrome aids in the assessment of tissue health and disease progression.