Live/Dead Staining is a technique used to differentiate between live and dead cells in a sample. It typically involves using fluorescent dyes that penetrate only dead cells, allowing researchers to visualize and quantify cell viability under a microscope. Common dyes include propidium iodide for dead cells and calcein-AM for live cells. This method is widely used in various fields, including cell biology, microbiology, and pharmaceutical research. By assessing cell viability, scientists can evaluate the effects of treatments, understand cellular responses, and improve the development of drugs and therapies.