In Vitro Methods refer to techniques that study biological processes outside of a living organism, typically in a controlled laboratory environment. These methods often involve using cells, tissues, or organs cultured in petri dishes or test tubes. Researchers can manipulate conditions to observe how cells respond to various stimuli, drugs, or environmental factors. These methods are essential in fields like pharmacology, toxicology, and biomedical research. They allow scientists to conduct experiments without the ethical concerns associated with animal testing. Additionally, in vitro studies can provide valuable insights into disease mechanisms and help in the development of new therapies.