High-Performance Liquid Chromatography, or HPLC, is a technique used to separate, identify, and quantify components in a mixture. It works by passing a liquid sample through a column filled with a solid material, allowing different substances to move at different rates. This separation helps scientists analyze complex mixtures in various fields, including chemistry, biology, and pharmaceuticals. HPLC is widely used in laboratories for quality control and research purposes. It can analyze small quantities of substances, making it valuable for detecting impurities in drugs or studying biochemical compounds. The method is known for its accuracy, speed, and ability to handle a variety of samples.