Fluorophores are special molecules that can absorb light at one wavelength and then emit light at a different wavelength. This property makes them useful in various scientific fields, especially in biotechnology and medical imaging. When excited by a light source, fluorophores glow, allowing researchers to visualize and track biological processes in cells and tissues. These glowing molecules are often used in fluorescence microscopy, a technique that helps scientists see structures within cells. By attaching fluorophores to specific proteins or DNA, researchers can study how these components behave in real-time, providing valuable insights into cell biology and disease mechanisms.