Fluorometric assays are laboratory techniques used to measure the concentration of specific substances by detecting fluorescence. These assays utilize fluorescent dyes or probes that emit light when excited by a specific wavelength. The intensity of the emitted light is proportional to the amount of the target substance, allowing for quantitative analysis. These assays are widely used in various fields, including biochemistry, molecular biology, and clinical diagnostics. They offer advantages such as high sensitivity, rapid results, and the ability to analyze multiple samples simultaneously. Common applications include measuring enzyme activity, detecting nucleic acids, and assessing cellular processes.