Fluorescence imaging is a technique used to visualize and study biological samples by detecting the light emitted from fluorescent molecules. When these molecules are excited by a specific wavelength of light, they emit light at a longer wavelength, allowing researchers to observe structures and processes within cells and tissues. This method is widely used in fields like cell biology, molecular biology, and medical diagnostics. The technique often involves the use of fluorescent dyes or proteins, such as GFP (green fluorescent protein), which can be attached to specific targets within a sample. By capturing images of the emitted fluorescence, scientists can gain insights into cellular functions, interactions, and the localization of proteins, enhancing our understanding of complex biological systems.