Droplet Digital PCR (ddPCR) is a highly sensitive technique used to quantify DNA or RNA. It works by partitioning a sample into thousands of tiny droplets, each acting as an individual reaction chamber. This allows for the detection of rare genetic variants and precise measurement of nucleic acid concentrations. In ddPCR, each droplet undergoes a separate polymerase chain reaction, amplifying the target DNA. After amplification, the droplets are analyzed to determine how many contain the target sequence. This method provides absolute quantification without the need for standard curves, making it valuable in research and clinical diagnostics.