Dark Field Microscopy
Dark Field Microscopy is a technique used in optical microscopy that enhances the contrast of unstained samples. It works by illuminating the specimen with light that is scattered rather than transmitted, allowing the viewer to see details that would otherwise be invisible against a bright background. This method is particularly useful for observing live cells and microorganisms.
In Dark Field Microscopy, a special condenser is used to direct light at an angle, creating a dark background. Only the light scattered by the specimen enters the objective lens, making the sample appear bright against the dark field. This technique is valuable in biological and materials science research for studying fine structures.