Limb formation is a complex biological process that occurs during the development of vertebrate embryos. It involves the growth and differentiation of cells to create the structures of the arms and legs. This process is guided by specific genes and signaling pathways, which ensure that limbs develop in the correct size and shape. During limb formation, mesoderm and ectoderm tissues interact to form limb buds. These buds eventually develop into the bones, muscles, and skin of the limbs. Key proteins, such as Sonic Hedgehog and Fibroblast Growth Factor, play crucial roles in regulating this development, ensuring proper limb patterning and growth.