Hexokinase III is an enzyme that plays a crucial role in glucose metabolism. It catalyzes the phosphorylation of glucose to form glucose-6-phosphate, which is an important step in the process of converting glucose into energy. This enzyme is found in various tissues, including the brain and skeletal muscle, and helps regulate blood sugar levels. Unlike other hexokinases, hexokinase III has a lower affinity for glucose, meaning it is less active when glucose levels are high. This characteristic allows it to function effectively in tissues where glucose is abundant, ensuring that energy production is balanced according to the cell's needs.