Fructose-2,6-bisphosphatase is an enzyme that plays a crucial role in regulating glucose metabolism. It is primarily involved in the process of gluconeogenesis, which is the production of glucose from non-carbohydrate sources. This enzyme helps control the levels of fructose-2,6-bisphosphate, a key molecule that influences the activity of other enzymes in the metabolic pathway. The activity of fructose-2,6-bisphosphatase is regulated by various factors, including hormonal signals like insulin and glucagon. When insulin levels are high, the enzyme's activity decreases, promoting the use of glucose for energy. Conversely, when glucagon is present, the enzyme's activity increases, facilitating the production of glucose during fasting or low-energy states.