Fluorometric assays are laboratory techniques used to measure the concentration of specific substances by detecting their fluorescence. These assays involve exciting a sample with light at a particular wavelength, causing the sample to emit light at a different wavelength. The intensity of the emitted light is proportional to the amount of the target substance present, allowing for quantitative analysis. These assays are commonly used in various fields, including biochemistry, molecular biology, and clinical diagnostics. They can detect a wide range of molecules, such as proteins, nucleic acids, and small metabolites, making them valuable tools for research and medical applications.