Fluorescence lifetime imaging (FLIM) is a technique used to measure the time that fluorescent molecules emit light after being excited by a light source. This method provides information about the local environment of the fluorescent molecules, which can reveal details about cellular processes and interactions. FLIM is particularly useful in biological research, as it allows scientists to visualize and study living cells in real-time. In FLIM, the emitted light is captured using specialized detectors, and the lifetime of the fluorescence is analyzed to create images. This technique can be combined with other imaging methods, such as confocal microscopy, to enhance the resolution and provide more detailed information about the sample being studied.