The broth dilution method is a laboratory technique used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent against a specific microorganism. In this method, a series of test tubes or wells containing a nutrient broth are prepared, each with a different concentration of the antimicrobial substance. The microorganism is then added to each tube, and after incubation, the growth is assessed to identify the lowest concentration that inhibits visible growth. This method is commonly used in microbiology to guide treatment decisions for infections caused by bacteria, fungi, or other pathogens. By identifying the MIC, healthcare professionals can select the most effective antibiotic or antifungal therapy for patients, ensuring better outcomes and minimizing the risk of resistance development.