Alpha-ketoglutarate dehydrogenase is an important enzyme in the Krebs cycle, which is a key metabolic pathway for energy production in cells. This enzyme catalyzes the conversion of alpha-ketoglutarate into succinyl-CoA, while also producing NADH from NAD+. This reaction is crucial for the proper functioning of cellular respiration. The enzyme is a multi-subunit complex that requires several cofactors, including thiamine and lipoic acid, to function effectively. Deficiencies or malfunctions in alpha-ketoglutarate dehydrogenase can lead to metabolic disorders and affect energy production, highlighting its significance in cellular metabolism.