In "Toy Story 3," the beloved characters from the previous films face a new challenge as their owner, Andy, prepares to leave for college. The toys, including Woody, Buzz Lightyear, and Jessie, worry about their future and what will happen to them once Andy no longer needs them. When they accidentally end up at a daycare center, they encounter new toys and a different environment. However, they soon realize that not all toys are friendly, leading to a thrilling adventure as they work together to find their way back home to Andy.