T.U.F.F. stands for "Turbo Undercover Fighting Force," a fictional organization featured in the animated television series T.U.F.F. Puppy. The show follows a dog named Dudley Puppy, who becomes a secret agent working for T.U.F.F. to protect the city of Petropolis from various villains. The series showcases Dudley's adventures alongside his partner, Kitty Katswell, and their boss, Chief. Together, they face off against a range of quirky antagonists, including the evil D.O.O.M. (Diabolical Order of Mayhem). The show combines humor and action, appealing to both children and families.