SYBR Green
SYBR Green is a fluorescent dye commonly used in molecular biology for detecting and quantifying DNA during polymerase chain reaction (PCR) processes. It binds to double-stranded DNA, emitting a bright green fluorescence when exposed to specific wavelengths of light. This property allows researchers to monitor the amplification of DNA in real-time.
The use of SYBR Green is popular due to its simplicity and cost-effectiveness compared to other detection methods. However, it is important to note that it can bind to non-specific DNA as well, which may lead to false positives. Proper experimental controls are essential for accurate results.