Purkinje fibers are specialized muscle fibers located in the heart's conduction system. They play a crucial role in transmitting electrical impulses that coordinate heartbeats. These fibers ensure that the ventricles contract in a synchronized manner, allowing for efficient blood pumping. These fibers are found in the inner ventricular walls of the heart and are part of the Bundle of His, which branches into the right and left bundle branches. Their unique structure allows for rapid conduction of electrical signals, making them essential for maintaining a regular heart rhythm and overall cardiovascular health.