Metabolite profiling is a scientific technique used to analyze the small molecules, or metabolites, present in biological samples. This process helps researchers understand the metabolic processes occurring within cells, tissues, or organisms. By identifying and quantifying these metabolites, scientists can gain insights into health, disease, and the effects of drugs or environmental changes. The technique often employs methods like mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy to detect and measure metabolites. Applications of metabolite profiling include biomedical research, pharmacology, and nutritional studies, making it a valuable tool in various fields of science and medicine.