Masson's Trichrome is a histological staining technique used to differentiate between collagen and muscle fibers in tissue samples. It employs three different dyes: aniline blue, acid fuchsin, and yellowish-orange, allowing for clear visualization of connective tissue, muscle, and cytoplasm. This method is particularly useful in identifying fibrosis and other tissue changes. The staining process involves fixing the tissue, followed by a series of steps that include staining, washing, and dehydration. Masson's Trichrome is commonly used in pathology to assess various conditions, including liver disease and cardiac fibrosis, providing valuable insights into tissue structure and health.