The Lost Colony of Roanoke refers to a group of English settlers who established a colony on Roanoke Island, North Carolina, in 1587. Led by John White, the colony was intended to be a permanent settlement. However, when White returned from England in 1590, he found the colony deserted, with no sign of the settlers. The only clue left behind was the word “Croatoan” carved into a tree. This has led to various theories about the fate of the colonists, including assimilation with local Native American tribes or relocation. Despite numerous investigations, the mystery of the Lost Colony remains unsolved.