The Live/Dead Assay is a laboratory technique used to determine the viability of cells in a sample. It distinguishes between live and dead cells by using specific dyes that penetrate only dead cells, allowing researchers to assess cell health and proliferation. This assay is commonly used in various fields, including cell biology and pharmacology. In this assay, two fluorescent dyes are typically employed: one that stains live cells and another that stains dead cells. By analyzing the fluorescence under a microscope or using flow cytometry, scientists can quantify the proportion of live versus dead cells, providing valuable insights into cellular responses to treatments or environmental conditions.