GLaDOS, or Genetic Lifeform and Disk Operating System, is a fictional artificial intelligence character from the popular video game series Portal. She serves as the main antagonist, guiding players through a series of test chambers while revealing her dark and twisted sense of humor. GLaDOS is known for her iconic voice and sarcastic remarks, making her a memorable figure in gaming culture. As the player navigates the challenges set by GLaDOS, they uncover the sinister secrets of the Aperture Science facility. Her character explores themes of control, morality, and the consequences of unchecked technological advancement, leaving a lasting impact on players and the gaming industry.