Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful imaging technique that measures the time a fluorescent molecule remains in an excited state before emitting light. This method provides detailed information about the local environment of the fluorescent molecules, which can reveal insights into biological processes and molecular interactions. FLIM works by using a pulsed light source to excite fluorescent dyes or proteins, capturing the emitted light with high temporal resolution. By analyzing the decay time of the fluorescence, researchers can obtain images that reflect variations in molecular dynamics, making FLIM valuable in fields like cell biology and biomedical research.