Fleming's Test is a simple laboratory procedure used to identify the presence of penicillin in a sample. It involves placing a sample on an agar plate that has been inoculated with Staphylococcus aureus, a type of bacteria. If penicillin is present, a clear zone will appear around the sample, indicating that the bacteria cannot grow in that area due to the antibiotic's effect. This test is named after Alexander Fleming, who discovered penicillin in 1928. It is a crucial method in microbiology for assessing the effectiveness of antibiotics and ensuring proper treatment for bacterial infections.