In "Despicable Me 2," former supervillain Gru is now a devoted father to his three adopted daughters, Margo, Edith, and Agnes. He is trying to lead a normal life while running a jam business. However, he is recruited by the Anti-Villain League to help stop a new villain who has stolen a powerful serum that can turn animals into monstrous versions of themselves. To assist him, Gru teams up with Lucy Wilde, a quirky agent from the Anti-Villain League. Together, they investigate the villain's lair and uncover a plot that threatens the world. Throughout the adventure, Gru learns about love and family while facing challenges that test his skills and resolve.