DNA Ligase is an essential enzyme in the process of DNA replication and repair. It functions by joining together the sugar-phosphate backbones of DNA strands, sealing any nicks or gaps that may occur. This action is crucial for maintaining the integrity of the genetic material during cell division and in response to DNA damage. In addition to its role in natural cellular processes, DNA Ligase is widely used in molecular biology techniques, such as cloning and genetic engineering. By facilitating the joining of DNA fragments, it allows scientists to create recombinant DNA molecules, which are vital for research, medicine, and biotechnology applications.