A broth culture is a method used in microbiology to grow and maintain microorganisms, such as bacteria or yeast, in a liquid nutrient medium. This technique allows for the multiplication of cells, making it easier to study their characteristics and behaviors. The broth typically contains essential nutrients, such as sugars, amino acids, and vitamins, which support the growth of the microorganisms. In a laboratory setting, scientists often use broth cultures to isolate specific strains of microorganisms for research or diagnostic purposes. By inoculating the broth with a small number of cells, they can observe growth patterns and test the effects of various conditions on the organisms. This method is essential for understanding the biology of microbes and their potential applications in fields like medicine and biotechnology.