Alpha-enolase is an enzyme that plays a crucial role in the glycolysis pathway, which is the process cells use to convert glucose into energy. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, facilitating the production of ATP, the energy currency of the cell. This enzyme is found in various tissues, including muscle and brain, highlighting its importance in energy metabolism. In addition to its metabolic functions, alpha-enolase has been identified as a potential biomarker for certain diseases, including cancer and neurodegenerative disorders. Its presence in the bloodstream can indicate cellular stress or damage, making it a subject of interest in medical research for diagnostic purposes.